Additional linkage tests of non-waxy (Waxy1) reciprocal translocations involving chromosome 9 at the MGCSC --Jackson, JD, Stinard, P, Zimmerman, S Approximately one acre each year is devoted to the propagation of the large collection of A-A translocation stocks. In this collection is a series of Waxy1-linked translocations that are used for mapping unplaced mutants. Each translocation is maintained in separate M14 and W23 inbred backgrounds, which are crossed, together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and can now supply good sources proven by linkage tests to include the correct translocated chromosomes.

Previously we reported the linkage results for some of these stocks (MNL72:79-81; MNL73:86-88; MNL74:67; MNL75:67). Below is a summary of additional translocation stocks we have completed testing.

Table 1. Wx1 T1-9(8389) (1L.74; 9L.13)

A) The F1 source showed linkage of wx1 with bm2:

Three point linkage data for bm2-T1-9(8389)Wx1
Modified backcross: bm2 N wx1 x [bm2 N wx1 x Bm2 T1-9(8389) Wx1]

source: 87-958-2^F1
Region Phenotype No. Totals
0 + T Wx 102  
  bm N wx 78 180
1 + N wx 46  
  bm T Wx 40 86
2 + T wx 11  
  bm N Wx 6 17
1+2 + N Wx 13  
  bm T wx 25 38
% recombination bm2-T = 38.6+2.7
% recombination T-wx1 =17.1+2.1
% recombination bm2-wx1 = 55.8+2.8

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Table 2. Wx1 T2-9d (2L.83; 9L.27)

A) The F1 source showed linkage of wx1 with ch1:

Three point linkage data for Ch1-T2-9dWx1
Modified backcross: ch1 N wx1 x [Ch1 N wx1 x ch1 T2-9d Wx1]

source: 98-842-1^F1
Region Phenotype No. Totals
0 + T Wx 80  
  Ch N wx 78 158
1 + N wx 41  
  Ch T Wx 49 90
2 + T wx 13  
  Ch N Wx 6 19
1+2 + N Wx 2  
  Ch T wx 21 23
% recombination ch1-T =39.0+2.9
% recombination T-wx1=14.5+2.1
% recombination ch1-wx1=53.4+2.9

B) The M14 source showed linkage of wx1 with ch1:

Three point linkage data for Ch1-T2-9dWx1
Modified backcross: ch1 N wx1 x [Ch1 N wx1 x ch1 T2-9d Wx1]

source: 75-242-2; fr.95W-257^M14
Region Phenotype No. Totals
0 + T Wx 110  
  Ch N wx 109 219
1 + N wx 65  
  Ch T Wx 66 131
2 + T wx 10  
  Ch N Wx 7 17
1+2 + N Wx 9  
  Ch T wx 9 18
% recombination ch1-T =38.7+2.5
% recombination T-wx1=9.1+1.5
% recombination ch1-wx1=47.8+2.5

C) The W23 source showed linkage of wx1 with ch1:

Three point linkage data for Ch1-T2-9dWx1
Modified backcross: ch1 N wx1 x [Ch1 N wx1 x ch1 T2-9d Wx1]

source: 93W-1439-3^W23
Region Phenotype No. Totals
0 + T Wx 121  
  Ch N wx 97 218
1 + N wx 35  
  Ch T Wx 59 94
2 + T wx 21  
  Ch N Wx 2 23
1+2 + N Wx 3  
  Ch T wx 13 16
% recombination ch1-T =31.3+2.5
% recombination T-wx1=11.1+1.7
% recombination ch1-wx1=42.5+2.6

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Table 3. Wx1 T2-9c (2S.49; 9S.33)

A) The F1 source showed linkage of wx1 with lg1gl2.

Three point linkage data for lg1 gl2-Wx1 T2-9c
Testcross: lg1 gl2 wx1 N x [lg1 gl2 wx1 N x Lg1 Gl2 Wx1 T2-9c]

source: 87-962 x 960 ^F1
Region Phenotype No. Totals
0 lg gl wx 429  
  + + Wx 617 1046
1 + gl wx 158  
  lg + Wx 217 375
2 + + wx 82  
  lg gl Wx 88 170
1+2 lg + wx 12  
  + gl Wx 12 24
% recombination lg1-g12 =24.7+1.1
% recombination gl2-wx1=12.0+0.8
% recombination lg1-wx1=36.7+1.2

B) The F1 source showed linkage of wx1 with v4.

Two point linkage data for v4-Wx1 T2-9c
Testcross: [V4 Wx1 T2-9c x v4 wx1 N] x v4 wx1 N

source: 87-962 x 960^F1
Region Phenotype No. Totals
0 + Wx 381  
  v wx 333 714
1 v Wx 212  
  + wx 120 332
% recombination v4-wx1=31.7+1.4

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Table 4. Wx1 T3-9(8447) (3S.44; 9L.14)

A) The F1 source showed linkage of wx1 with gl6.

Two point linkage data for gl6-Wx1 T3-9(8447)
Testcross: [Gl6 Wx1 T3-9(8447) x gl6 wx1 N] x gl6 wx1 N

source: 87-974 x 972^F1
Region Phenotype No. Totals
0 + Wx 1688  
  gl wx 1675 3363
1 gl Wx 122  
  + wx 121 243
% recombination gl6-wx1=6.7+0.4

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Table 5. Wx1 T4-9(5657) (4L.33; 9S.25)

A) The F1 source showed linkage of wx1 with gl4:

Two point linkage data for gl4-Wx1 T4-9(5657)
Testcross: [Gl4 Wx1 T4-9(5657) x gl4 wx1 N] x gl4 wx1 N

source: 87-995 x 992^F1
Region Phenotype No. Totals
0 + Wx 1724  
  gl wx 1569 3293
1 gl Wx 16  
  + wx 12 28
% recombination gl4-wx1= 0.8+0.2

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Table 6. Wx T6-9b (6L.10; 9S.37)

A) The M14 sources showed linkage of wx1 with y1:

Two point linkage data for y1-Wx1 T6-9b
Testcross: [y1 Wx1 T6-9b x Y1 wx1 N] x y1 wx1 N

source: 96-840-4^M14
Region Phenotype No. Totals
0 y Wx 2071  
  + wx 3026 5097
1 + Wx 397  
  y wx 34 431
% recombination y1-wx1= 7.8+0.4

source: 96-839-10^M14
Region Phenotype No. Totals
0 y Wx 1280  
  + wx 2042 3322
1 + Wx 251  
  y wx 13 264
% recombination y1-wx1= 7.4+0.4

B) The W23 sources gave varying linkage values of wx1 with y1 depending on whether Translocation was transmitted through male or female :

Two point linkage data for y1-Wx1 T6-9b
1) Testcross: [Y1 wx1 N x y1 Wx1 T6-9b] x y1 wx1 N

source: 97-1271-1^W23
Region Phenotype No. Totals
0 y Wx 941  
  + wx 1404 2345
1 + Wx 246  
  y wx 29 275
% recombination y1-wx1=10.5+0.6

source: 97-1271-2^W23
Region Phenotype No. Totals
0 y Wx 1373  
  + wx 1822 3195
1 + Wx 367  
  y wx 37 404
% recombination y1-wx1=11.2+0.5

source: 97-1273-1^W23
Region Phenotype No. Totals
0 y Wx 4003  
  + wx 5294 9297
1 + Wx 883  
  y wx 99 982
% recombination y1-wx1=9.6+0.3

2) Testcross: y1 wx1 N x [Y1 wx1 N x y1 Wx1 T6-9b]

source: 97-1271-1^W23
Region Phenotype No. Totals
0 y Wx 228  
  + wx 229 457
1 + Wx 5  
  y wx 6 11
% recombination y1-wx1=2.4+0.7

source: 97-1271-2^W23
Region Phenotype No. Totals
0 y Wx 388  
  + wx 370 758
1 + Wx 21  
  y wx 15 36
% recombination y1-wx1=4.5+0.7

source:97-1273-1^W23
Region Phenotype No. Totals
0 y Wx 1180  
  + wx 1125 2305
1 + Wx 40  
  y wx 14 54
% recombination y1-wx1=2.3+0.3
 
 


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