In the 1999 Johnston, IA, nursery, we planted an ms24 F2 segregating
family to determine the chromosome arm map location of this mutant. Leaf
punches were taken from 24 male-sterile plants and from 24 male-fertile
plants for DNA isolation. 96 SSR markers, dispersed throughout the genome,
were used to genotype these samples. Three
markers on chromosome 10 showed linkage with the ms24 phenotype.
Using markers bngl1655 and phi059, four recombinant alleles
were found for each in 19 mutant plants (38 alleles) and 1 recombinant
allele was found with marker bngl1079 in 17 mutant plants (34 alleles).
This data indicates that ms24 maps to chromosome 10 near the centromere.
Return to the MNL 76 On-Line Index
Return to the Maize Newsletter Index
Return to the Maize Genome Database Page