To date, we have cloned sequences flanking 64 Ac elements and confirmed 39 as linked to an active, germinally-inherited Ac element. We have placed 18 on the genetic map, and are continually cloning, mapping, and generating additional active Acís at unique locations throughout the genome (Figure 1).
In addition to the development of the technology and protocols for regional mutagenesis, we plan to calculate the efficiency of localized Ac-mutagenesis. Utilizing several aAc lines positioned on chromosome 1, we are generating transpositions and screening the F2 progeny for Ac- induced mutations. This will allow us to determine the frequency of Ac-induced mutations and to examine the relationship between proximity of a target locus and the donor Ac line.
Finally, an important objective of this project is to develop a summer
genetics program involving senior researchers, graduate students, undergraduate
students, high school teachers, and high school students. This program
is designed to facilitate interactions between often isolated groups in
both research and education. It is our hope that this will give
researchers a chance to familiarize themselves with the use of these aAc
lines, while at the same time educating young students about maize genetics,
and increasing the public understanding of science. The Emerson Summer
Genetics Program officially began in June 2001. The participants included:
five high school students (local and national), one high school teacher,
three undergraduates (local and international), two post-docs, two graduate
students, and two senior researchers. The participants cooperated on various
projects in the field and lab and have developed a web site detailing the
program activities at: (http://bti.cornell.edu
the students participating in this program helped generate 3,000 transpositions
from Acís positioned on chromosome 1 that will be used in determining
the frequency of Ac-induced mutations.
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