Additional linkage tests of waxy1 marked reciprocal translocations at the MGCSC --Jackson, JD, Stinard, P, Zimmerman, S In the collection of A-A translocation stocks maintained at MGCSC is a series of waxy1-linked translocations that are used for mapping unplaced mutants. New wx1-linked translocations are being introduced into this series and we are in the process of converting each translocation to the inbred backgrounds M14 and W23. These inbred conversions are then crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and can now supply good sources proven by linkage tests to include the correct translocated chromosomes. Additional pedigree information on bad sources is available should anyone want to check on sources supplied to them previously by the Stock Center.

Previously, we reported the linkage results for some of these stocks (MNL 72:81-82; MNL 73:88-89; MNL 74:67-69; MNL 75:68-71; MNL 76:65-67). Below is a summary of additional translocation stocks we have completed testing. Additional translocation stocks will be tested as time allows.

Table 1. wx1 T2-9c (2S.49; 9S.33)

A) The M14 source showed linkage of wx1 with lg1 gl2.

3 point linkage data for lg1 gl2-wx1 T2-9c
Testcross: lg1 gl2 wx1 N x [lg1 gl2 Wx1 N x Lg1 Gl2 wx1 T2-9c]

source:93-480-2 x SIB ^M14
Region Phenotype No. Totals
0 lg gl Wx 103  
  + + wx 83 186
1 + gl Wx 28  
  lg + wx 30 58
2 + + Wx 23  
  lg gl wx 23 46
1+2 lg + Wx 1  
  + gl wx 2 3
% recombination lg1-gl2 =20.8±2.4
% recombination gl2-wx1=16.7±2.2
% recombination lg1-wx1= 37.5±2.8

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Table 2. wx1 T3-9c (3L.09; 9L.12)

A) The M14 source showed linkage of wx1 with gl6.

2 point linkage data for gl6-wx1 T3-9c
Testcross: [Gl6 wx1 T3-9c x gl6 Wx1 N] x gl6 wx1 N

source:93-481-1 x SIB ^M14
Region Phenotype No. Totals
0 gl Wx 446  
  + wx 464 910
1 + Wx 25  
  gl wx 1 26
% recombination gl6-wx1=2.8±0.5

B) The W23 source showed linkage of wx1 with gl6.

2 point linkage data for gl6-wx1 T3-9c
Testcross: [Gl6 wx1 T3-9c x gl6 Wx1 N] x gl6 wx1 N

source:94-1893-2^W23
Region Phenotype No. Totals
0 gl Wx 669  
  + wx 683 1352
1 + Wx 29  
  gl wx 3 32
% recombination gl6-wx1=2.3±0.4

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Table 3. wx1 T9-10(8630) (9S.28; 10L.37)

A) Another new M14 crossover source showed linkage of wx1 with g1.

2 point linkage data for g1-wx1 T9-10(8630)
Testcross: [G1 wx1 T9-10(8630) x g1 Wx1 N ] x g1 wx1 N

source:99-1351-7c/o^M14
Region Phenotype No. Totals
0 g Wx 2098  
  + wx 2181 4279
1 + Wx 71  
  g wx 56 127
% recombination g1-wx1=2.9±0.3

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Table 4. wx1 T5-9(8386) (5L.87; 9S.13)

All Coop and Robertson sources showed no linkage with v2.
 
 


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