Additional linkage tests of non-waxy (Waxy1) reciprocal translocations involving chromosome 9 at the MGCSC --Jackson, JD, Stinard, P, Zimmerman, S Approximately 1 acre each year is devoted to the propagation of the large collection of A-A translocation stocks at the Maize Genetics Stock Center. In this collection is a series of Waxy1-linked translocations that are used for mapping unplaced mutants. Each translocation is maintained in separate M14 and W23 inbred backgrounds which are crossed together to produce vigorous F1's to fill seed requests. Over the years, pedigree and classification problems arose during the propagation of these stocks. We have been able to sort through the problem ones, and can now supply good sources proven by linkage tests to include the correct translocated chromosomes.

Previously, we reported linkage results for some of these stocks (MNL 72:79-81; MNL 73:86-88; MNL 74:67; MNL 75:67; MNL 76:67-68). Below is a summary of additional translocation stocks we have completed testing.

Table 1. Wx1 T3-9c (3L.09; 9L.12)

A) The M14 source showed linkage of wx1 with gl6.

2 point linkage data for gl6-Wx1 T3-9c
Testcross: [Gl6 Wx1 T3-9c x gl6 wx1 N] x gl6 wx1 N

source:82-198-5^M14
Region Phenotype No. Totals
0 + Wx 1697  
  gl wx 1609 3306
1 gl Wx 18  
  + wx 27 45
% recombination gl6-wx1=1.3±0.2

B) The W23 source showed linkage of wx1 with gl6.

2 point linkage data for gl6-Wx1 T3-9c
Testcross: [Gl6 Wx1 T3-9c x gl6 wx1 N] x gl6 wx1 N

source:2000-1411-1^W23
Region Phenotype No. Totals
0 + Wx 1414  
  gl wx 1222 2636
1 gl Wx 16  
  + wx 21 37
% recombination gl6-wx1=1.4±.0.2

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Table 2. Wx1 T6-9(4778) (6S.80; 9L.30)

A) The F1 source showed linkage of wx1 with hcf26.

2 point linkage data for hcf26-Wx1 T6-9(4778)
Testcross: [Hcf26 Wx1 T6-9(4778) x hcf26 wx1 N] x hcf26 wx1 N

source:87-1023 x 1024^F1
Region Phenotype No. Totals
0 + Wx 404  
  hcf wx 441 845
1 hcf Wx 40  
  + wx 31 71
% recombination hcf26-wx1=7.8±0.9

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Table 3. Wx1 T5-9(8386) (5L.87; 9S.13)

All Coop and Robertson sources showed no linkage with v2.

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Table 4. Wx1 T6-9(8768) (6L.89; 9S..61)

The F1; M14 and W23 sources showed no linkage with sm1.
 
 


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