BERKELEY, CALIFORNIA
University of California-Berkeley
Molecular characterization of mutations resulting in a liguleless1 phenotype --Braun, DM, Freeling, M In our studies on liguleless1 (lg1) we have characterized many independent mutations with a liguleless phenotype. These mutants were obtained from the Maize Coop Genetic Stock Center, sent to us by other collaborators, or identified in the Freeling lab. All were determined to be alleles of lg1 by complementation testing the new liguleless mutants to the lg1-Reference allele. All of the F1 progeny showed a mutant phenotype. The F1 were self-fertilized and the F2 displayed a 100% mutant phenotype indicating that the new mutant being tested was allelic to lg1. All of the alleles have a phenotype indistinguishable from the reference allele with the exception of lg1-N2375 which has a much weaker phenotype. The nature of the lesion in the lg1 gene was determined for many of the alleles (Table 1). To characterize each mutation, a combination of Southern blot analysis, PCR amplification and DNA sequence analysis of the coding region of lg1 was performed. Note that for three alleles we were unable to find any changes in the coding region; these alleles likely represent cis regulatory mutations.

Table 1. Molecular alterations in various lg1 alleles.
 
Allele
Alteration
lg1-Reference
Deletion of entire gene
lg1-64-4
Deletion of entire gene
lg1-6198
Deletion of entire gene
lg1-K16
Deletion of entire gene
lg1-Ds
Deletion of entire gene
lg1-PI200299
268 bp deletion of promoter, transcription & translation start sites
lg1-m1
Ac insertion in intron1
lg1-MTM
Mu insertion in 5 end
lg1-PI262493
Tourist insertion in exon2
lg1-TaiTaiTaSarga
4 bp insertion in exon2
lg1-ZCxGRB
Insertion in 5 end
lg1-128
Insertion in 5 end
lg1-340
Insertion in 5 end
lg1-656
Insertion in 5 end
lg1-N2375
V173M mutation in DNA binding domain
lg1-m2
unknown
lg1-ems
unknown
lg1-56-30375
unknown

 


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