University of Toledo1
Ball State University2
Institute of Cytology and Genetics4
Tripsacum stage- and ploidy-specific floral cDNA libraries
--Goldman, SL1, Blakey, CA2, Frutiger, K1,
Dewald, CL3, Sokolov, VA4
Using the pilot project designed at Ball State University (BSU), Muncie,
IN, by Blakey et al. (MNL 77), a set of five out of six possible stage-specific
cDNA libraries were constructed at the Univ. of Toledo using the SMART™
PCR cDNA Synthesis Kit (CLONTECH Cat# K1052-1) at the University of Toledo.
The original RNA was isolated in 1998 by Blakey et al. (MNL 77) on-site
in Woodward, OK, according to the Trizol™ RNA isolation protocol
provided by Sigma. The materials were quantitated at BSU and half were
shipped to the U. Toledo. Each library was labeled according to the staged
tissue from which the RNA was isolated: "E" early diploid (E2) or early
tetraploid (E4), "M" middle diploid (M2), and "L" late diploid (L2) or
late tetraploid (L4). The only library that was not obtained at this time
was the "M" middle tetraploid (M4) library. Twenty-five cDNAs from these
libraries were shipped to the DNA Core Facility, Ohio State University,
Columbus, OH, for sequencing.
Sequence data analysis of clones from the E2 and M2 libraries indicates
that the libraries include genes that function as a serine threonine protein
kinase and ribosomal protein S1Sa mRNA respectively. Clones from the E4
and L2 libraries have resulted in chloroplast gene sequences, while a clone
from the L4 library was considered novel at the time of this article.
Please Note: Notes submitted to the Maize Genetics Cooperation Newsletter may be cited only with consent of the authors.
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