A first report of mutants mapped using SSR markers at the University of Missouri --Carson, CB, Robertson, J, Coe, EH, Jr. We have mapped numerous maize mutants with SSR markers under the Maize Mapping Project (NSF Plant Genome Grant DBI 9872655). This note presents a portion of the results, for genes of visible mutants. Our purpose has been to identify linkage between SSR loci and mutants from the large maize collection, mainly to accelerate the discovery process. One of the goals is to map as many mutants as possible to approximate location, but not to attempt high-resolution mapping. All mutants were mapped in F2 families that were created from crosses between a parent carrying the mutant allele of interest crossed to either: A619, A632, B73, or Mo17. Each F2 was grown and samples collected based on the stage of development of the mutant phenotype. Classification of mutants for mapping:

1) Kernel, 2) Seedling, 3) Adult plant

For kernel mutants that affect the endosperm, endosperm samples were obtained from maturing F2 kernels on selfed F1 ears before desiccation. For viviparous mutants, the embryo was sampled. Samples of seedling mutants were obtained from F2 seed planted in dense cultures in a sandbench or a groundbed. The plant mutant class has the broadest range of phenotype and samples were obtained from F2 plants growing in larger cultures in the field. Genes of both recessive and dominant mutants were mapped successfully. Recessive mutants homozygous are mutant, segregating are normal

Dominant mutants homozygous are normal, segregating are mutant

Critical samples of individuals were obtained and placed in 96-well plates. To analyze up to 48 strands for crossing over, up to 24 individual homozygous samples were obtained. Despite attempts to grow enough F2 for samples, there were many cases of less than 24 definite homozygous samples and we collected only as many as possible. For control, an accompanying 8 segregating individuals were collected. To examine for both polymorphism of SSR markers in any F2, and linkage between polymorphic SSR markers and the gene of interest, we first made pools of the homozygous samples to compare with pools of the segregating samples. Based on prior map information for a given mutant, if any, a set of informative SSR marker primer pairs was set up to screen the pools broadly for linkage. For mutant loci that have been previously placed to the correct chromosome, chromosome specific sets of SSR markers were utilized. Otherwise, we used markers that span the whole genome to map unplaced mutants.

The pools were examined for SSR marker polymorphism and linkage using PCR followed by agarose gel electrophoresis. Images of ethidium bromide stained gels using ultraviolet light and an orange filter were examined to identify the band patterns. The pattern of the bands relates to the amount of DNA in the band produced by one allele versus the other. When the polymorphic SSR band pattern of a homozygous pool is similar to the pattern of the segregating pool, there is no linkage. However, when there is linkage between the gene that is homozygous in the pool and a particular SSR allele, the pattern of band accumulation in the homozygous pool is reciprocal to the segregating pool. Greater differences in band patterns indicate tighter linkage. Many times SSR primers might favor one allele over another causing greater accumulation of the DNA in that band; but, when this is taken into account, the analysis is reliable.

Initially, most pool analysis was followed up by a fingerprint analysis on the individuals from the homozygous pool using the polymorphic, linked SSR markers detected in the pool experiments. This provided validation and established linkage distance in map units. Later, we relied more on the pool experiments to report linkage.

The table presents the locus and variation names of the mutants studied as they appeared in the literature (MaizeDB) prior to SSR mapping. It also shows any prior genetic linkage information for the locus. Next, we report the SSR markers that are linked to the locus and the map information for those SSRs. The results from mapping using pools are reported as associations. When individual samples were analyzed the recombination percentage is reported. Then we readdressed the location and name of the locus. Next, we studied the available map information (using MaizeDB) to determine whether the locus is unique from other mutants having similar phenotypes or not. If the new locus is distinct from other mutants, the name is changed (the star is dropped and the next available number is assigned relative to other mutant loci with the same phenotype name). Most often there is some other mutant that is also linked to the locus we mapped and the two cannot be distinguished with the available map data. These are then assigned as candidates for allelism tests in the future. These decisions have been greatly facilitated by additional evaluation from Phil Stinard and Marty Sachs at the Maize Genetics Cooperation Stock Center.
 
 
 
COOP Stock # "S, K, or P" VARIANT MAPPED PRIOR BIN1 PRIOR BIN2 NEAR SSR SSR COORD IBM NEIGHBORS SSR BIN SSR RECOMB NEWBIN1 NEWBIN2 LOCUS NAME  NEW NAME? PROSPECTS (\=not) LOCATION CONFIRMED? ORDER
  S pg*-619 1S   umc1160 106.90 1.01 11% 1.01 1.02 pg*-N619   pg15 YES (pg*-N619 -11- umc1160)
Pheno   y-vp*-85-3101-36 unpl   umc1177 10.50 1.01   1.01 1.03 y-vp*-85-3101-36   vp5 NEW Associated in bulks
Pheno   y-vp*-85-3101-36 unpl   umc1073 206.70 1.03   1.01 1.03 y-vp*-85-3101-36   vp5 NEW Associated in bulks
Pheno   y-vp*-85-3101-36 unpl   umc1166 131.80 1.02   1.01 1.03 y-vp*-85-3101-36   vp5 NEW Associated in bulks
Pheno   y-vp*-8419 unpl   umc1177 10.50 1.01   1.01 1.02 y-vp*-8419   vp5 NEW Associated in bulks
Pheno   y-vp*-8419 unpl   umc1166 131.80 1.02   1.01 1.02 y-vp*-8419   vp5 NEW Associated in bulks
Pheno   y-vp*-8102 unpl   umc1166 131.80 1.02   1.01 1.03 y-vp*-8102   vp5 NEW Associated in bulks
  S sr1 1.02   umc1177  10.50 1.01 24% 1.02   sr1 --   YES umc1177 -24- sr1 -14- umc1166
  S sr1 1.02   umc1166  131.80 1.02 14% 1.02            
  S sr1 1.02   umc1568 140.00 1.02   1.02            
  S nec*-N495B 1S   umc1568 140.00 1.02 14% 1.02   blh*-N495B   blh1 YES (blh*-N495B -14- umc1568)
124D S v*-5588 1-   umc1166  131.80 1.02 25% 1.02   v*-5588   sr1 YES umc1166 (v*-5588 -14- umc1568) phi109275
124D S v*-5588 1-   umc1568  140.00 1.02 14% 1.02            
124D S v*-5588 1-   phi109275 262.20 1.03 12% 1.02            
124D S v*-5588 1-   umc1446  696.30 1.08 53% 1.02            
  S l16-N515 1S   phi109275 262.20 1.03 4% 1.03   l16 --   YES (l16 -4- phi109275)
  S wl*-N1930 1S?   umc1568 140.00 1.02 40% 1.04 1.05 wlu7 NEW w24,wlu5,l16,

l17

YES umc1568 phi109275 umc1144 -17- wlu7 -8- umc1395 umc1590 umc1035
  S wl*-N1930 1S?   phi109275 262.20 1.03 21% 1.04 1.05          
  S wl*-N1930 1S?   umc1144 334.00 1.04 17% 1.04 1.05          
  S wl*-N1930 1S?   umc1395 424.30 1.05 8% 1.04 1.05          
  S wl*-N1930 1S?   umc1590 469.20 1.06 17% 1.04 1.05          
  S wl*-N1930 1S?   umc1035 535.10 1.06 27% 1.04 1.05          
124C S w*-8345 1S   umc1590 469.20 1.06   1.05 1.06 w*-8345   w20, wlu7 YES Associated in bulks
124F S w*-4791 1L   umc1590 469.20 1.06   1.05 1.06 w20 NEW wlu7,\w24,wlu5,l16,l17 YES Associated in bulks
  S ppg*-N7A 1S?   umc1395 424.30 1.05 9% 1.05   ppg2 NEW pg15,pg16 YES (ppg2 -9- umc1395)
  S v*-N55 1L   umc1076  401.70 1.05 6% 1.05 1.06 v35 NEW f1,v22,pg16 YES umc1076 -6- v35 -10- umc1335 umc1446
  S v*-N55 1L   umc1335  551.00 1.06 10% 1.05 1.06          
  S v*-N55 1L   umc1446  696.30 1.08 30% 1.05 1.06          
124H S w*-8054 1.08   umc1493  400.60 1.05 21% 1.06 1.08 w24 NEW wlu5,l16,l17,

wlu7

YES umc1493 umc1335 -19- w24 -3- umc1446 umc1383
124H S w*-8054 1.08   umc1335  551.00 1.06 19% 1.06 1.08          
124H S w*-8054 1.08   umc1446  696.30 1.08 3% 1.06 1.08          
124H S w*-8054 1.08   umc1383  714.80 1.08 6% 1.06 1.08          
124I S v*-032-3 1   umc1396 500.50 1.06   1.06   v32 NEW v22,v35 YES Associated in bulks
120D P ms12 1   umc1245 647.70 1.07   1.07 1.08 ms12 --   YES Associated in bulks
  S rgd*-N766B 1L?   mmc0041 699.74 1.08 19% 1.08 1.09 rgd3 NEW   YES mmc0041 -19- rgd3 -21- bnlg1268
  S rgd*-N766B 1L?   bnlg1268 794.63 1.08-1.09 21% 1.07 1.11          
  S bl*-N43 1L   umc1335  551.00 1.06 28% 1.08 1.11 bl*-N43   pg16 YES umc1335 umc1446 -15- bl*-N43 -20- fdx3
  S bl*-N43 1L   umc1446  696.30 1.08 15% 1.08 1.11          
  S bl*-N43 1L   fdx3 952.80 1.11 20% 1.08 1.11          
  S l17-N544 1.09 1.10 bnlg1347 831.44 1.10 17% 1.09 1.11 l17 --   YES (l17 -17- bnlg1347)
129A S w18 1.09 1.10 umc1335 551 1.06 48% 1.09 1.10 w18 --   YES umc1335 umc1358 -27- w18 -30- fdx3
129A S w18 1.09 1.10 umc1358 584.7 1.07 27% 1.09 1.10          
129A S w18 1.09 1.10 umc1064 952.8 1.11 30% 1.09 1.10          
129A S w18 1.09 1.10 umc1335  551.00 1.06 29% 1.09 1.10 w18 --   YES umc1335 umc1358 (w18 -4- umc1306) bnlg1347
129A S w18 1.09 1.10 umc1358  584.70 1.07 25% 1.09 1.10          
129A S w18 1.09 1.10 umc1306  781.94 1.09 4% 1.09 1.10          
129A S w18 1.09 1.10 bnlg1347 831.00 1.10 27% 1.09 1.10          
  S ij2-N8 1.11 1.12 umc1331  951.30 1.11 0% 1.11   ij2 --   YES (ij2 -0- umc1331) -15- fdx3
  S ij2-N8 1.11 1.12 umc1064  952.80 1.11 15% 1.11            
  S spc*-N262A 1L 1.12 umc1331 951.30 1.11 12% 1.11   spc2 --   YES umc1331 (spc2 -7- phi064)
  S spc*-N262A 1L 1.12 umc1331 952.30 1.11 9% 1.11            
  S spc*-N262A 1L 1.12 phi064 957.80 1.11 7% 1.11            
  P Ts6 1.11   umc1421 880.40 1.11   1.11   ts6 --   YES Associated in bulks
  S nec*-N516B 2.02 2.04 umc1542 53.00 2.02 13% 2.01 2.02 nec4 --   YES (nec4 -13- umc1265 umc1542)
  S nec*-N516B 2.02 2.04 umc1265 73.10 2.02 13% 2.01 2.02          
  P d*-N155B 2S   umc1165 42.80 2.01 2% 2.01 2.02 d*-N155B   d5 YES (d*-N155B -2- umc1165) bnlg1036
  P d*-N155B 2S   bnlg1036 330.00 2.05 28% 2.01 2.02          
  K et*-N868A 2S?   umc1265 73.10 2.02 10% 2.01 2.03 et*-N868A   et2 YES (et*-N868A -10- umc1265)
  K rgh*-N1013A "2S?,3L?"   umc1542 53.00 2.02   2.01 2.02 rgh2 NEW et2 HIT  
  S gl2-N718 2.02   umc1552 46.30 2.02 15% 2.02   gl2 --   YES (gl2 -15- umc1552)
  S ws3-N453A 2.00 2.01 ole1 202.70 2.03 32% 2.00 2.01 ws3 --   YES (ws3 -32- ole1)
  S cb*-N652B 2S?   ole1 202.70 2.03   2.02 2.03 cb2 NEW al1 YES Associated in bulks
  K ptd*-N660E "2S?,3L?"   ole1 202.70 2.03   2.02 2.03 ptd*-N660E   et2 HIT Associated in bulks
  S wt*-N178C 2.04   ole1 202.70 2.03 4% 2.03 2.04 wt1   -- YES ole1 -4- wt1
  K fl*-N1426 2S   phi109642 214.20 2.03   2.03 2.04 fl*-N1426   fl1 YES Associated in bulks
  SP d*-N208B 2S   bnlg108 265.60 2.04   2.03 2.05 d*-N208B   d5 YES Associated in bulks
  S wt*-N136A 2S   phi402893 23.8 2.00 56% 2.04 2.05 wt1-N136A     YES phi402893 (wt1 -0- umc1026) -6- mmc0401
  S wt*-N136A 2S   umc1026 220.8 2.04 0% 2.04 2.05          
  S wt*-N136A 2S   mmc0401 315.9 2.05 6% 2.04 2.05          
  S wt*-N136A 2S   ole1 202.7 2.03-.04 19% 2.04 2.05 wt1-N136A     YES ole1 -19- wt1-13- bnlg108 umc1080
  S wt*-N136A 2S   bnlg108 265.6 2.04 13% 2.04 2.05          
  S wt*-N136A 2S   umc1080 337.2 2.06 13% 2.04 2.05          
224B S v*-5537 2-   umc1635 304.2 2.05 7% 2.04 2.06 v*-5537   v24,v4,wlv1 YES (umc1635 bnlg1887 -7- v*-5537) umc1042
224B S v*-5537 2-   bnlg1887 309.17 2.06 7% 2.04 2.06          
224B S v*-5537 2-   umc1042 408 2.07 17% 2.04 2.06          
224B S v*-5537 2-   bnlg2077 412.1 2.08 14% 2.04 2.06          
224B S v*-5537 2-   umc1604 449.1 2.08 21% 2.04 2.06          
224B S v*-5537 2-   bnlg1520 514.46 2.07-2.09 25% 2.04 2.06          
224B S v*-5537 2-   umc1256 516.5 2.09 26% 2.04 2.06          
224J S ij-mos*-7335 2-   phi109642 214.20 2.03-2.04 0% 2.04   sr5 NEW   YES phi109642 -0- sr5 -5- umc2032 bnlg108 umc1635
224J S ij-mos*-7335 2-   umc2032 254.50 2.04 5% 2.04            
224J S ij-mos*-7335 2-   bnlg108 265.60 2.04 9% 2.04            
224J S ij-mos*-7335 2-   umc1635  304.20 2.05 19% 2.04            
224J S ij-mos*-7335 2-   umc1080 337.20 2.05 27% 2.04            
224J S ij-mos*-7335 2-   umc1042 408.00 2.07 48% 2.04            
224J S ij-mos*-7335 2-   umc1604 449.10 2.08 43% 2.04            
224J S ij-mos*-7335 2-   umc1552 46.30 2.02 45% 2.04   sr5 NEW   YES umc1552 phi109642 -5- sr5 -3- umc2032 bnlg108 umc1635
224J S ij-mos*-7335 2-   phi109642 214.20 2.03-2.04 5% 2.04            
224J S ij-mos*-7335 2-   umc2032 254.50 2.04 3% 2.04            
224J S ij-mos*-7335 2-   bnlg108 265.60 2.04 5% 2.04            
224J S ij-mos*-7335 2-   umc1635  304.20 2.05 16% 2.04            
224J S ij-mos*-7335 2-   umc1042 408.00 2.07 21% 2.04            
224J S ij-mos*-7335 2-   mmc0191 415.80 2.07-.08 24% 2.04            
224J S ij-mos*-7335 2-   bnlg1520 514.46 2.07-2.09 34% 2.04            
215B S gl11 2.03   umc2032 254.5 2.04 28% 2.05 2.06 gl11 --   YES umc2032 umc1635 (bnlg1887 -18- gl11) mmc0401 umc1080
215B S gl11 2.03   umc1635 304.2 2.05 10% 2.05 2.06          
215B S gl11 2.03   bnlg1887 309.17 2.06 18% 2.05 2.06          
215B S gl11 2.03   mmc0401 315.9 2.05 18% 2.05 2.06          
215B S gl11 2.03   umc1080 337.2 2.06 25% 2.05 2.06          
  S l*-1940 2L   umc1635 304.2 2.05 6% 2.06 2.07 l18 --   YES umc1635 bnlg1887 -4- l18 -8- umc1042 bnlg2077 mmc0191
  S l*-1940 2L   bnlg1887 309.17 2.06 4% 2.06 2.07          
  S l*-1940 2L   umc1042 408 2.07 8% 2.06 2.07          
  S l*-1940 2L   bnlg2077 412.1 2.08 8% 2.06 2.07          
  S l*-1940 2L   mmc0191 415.8 2.07 8% 2.06 2.07          
  S w*-N77 2L   umc1004 338.90 2.06   2.06 2.08 w*-N77     YES Associated in bulks
  S w*-N77 2L   bnlg1887 309.17 2.06 13% 2.06 2.08 w*-N77   w3,w21 YES bnlg1887 -13- w*-N77 -24- umc1042 bnlg1520
  S w*-N77 2L   umc1042 408 2.07 24% 2.06 2.08          
  S w*-N77 2L   bnlg1520 514.46 2.07-2.09 22% 2.06 2.08          
  S w*-N77 2L   umc1256 516.5 2.09 22% 2.06 2.08          
  S v24-N424 2L   bnlg2077 412.1 2.08 9% 2.08   v24 --   YES bnlg2077 -9- v24 -9- mmc0191 bnlg1520 umc1256
  S v24-N424 2L   mmc0191 415.8 2.07 9% 2.08            
  S v24-N424 2L   bnlg1520 514.46 2.07-2.09 22% 2.08            
  S v24-N424 2L   umc1256 516.5 2.09 22% 2.08            
  K o*-N1195A 2L   umc1516 500.10 2.08 18% 2.08 2.09 o*-N1195A   dek16,\o*-999A YES (o*-N1195A -18- umc1516)
  K o*-N999A "2L?,1S?"   umc1604 449.10 2.08 20% 2.08   o*-N999A   dek16,\o*-N1195A HIT (o*-N999A -20- umc1604)
  S w*-N332 2L   umc1004 338.9 2.06 43% 2.08   w*-N332   w21 YES umc1004 bnlg2077 -11- w*-N332 -18- bnlg1520
  S w*-N332 2L   bnlg2077 412.1 2.08 11% 2.08            
  S w*-N332 2L   bnlg1520 514.96 2.07-2.09 18% 2.08            
  S w*-N1907 2L?   bnlg2077 412.1 2.08 0% 2.08 2.09 w*-N1907   w3 YES (w*-N1907 -0- bnlg1045 bnlg2077)
  S w*-N1907 2L?   bnlg1045 555.46 2.07 0% 2.08 2.09          
  K rgh*-N1112 3S   umc1527 248.60 3.04 12% 3.04 3.05 rgh*-N1112   ref1,dek5,

dek24

YES (rgh*-N1112 -12- umc1527)
  K smk*-N1230 3S   umc1527 248.60 3.04   3.04 3.05 smk*-N1230   ref1,dek5,

dek24

YES Associated in bulks
329D   yd2 3.06   umc1102 282.10 3.05 26% 3.06   yd2 --   YES umc1102 mmc0022 (umc1266 -8- yd2)
329D   yd2 3.06   mmc0022 285.00 3.05 24% 3.06            
329D   yd2 3.06   umc1266 358.30 3.06 8% 3.06            
329D   yd2 3.06   bnlg1754 635.00 3.09 34% 3.06            
  P d*-N282 3L   umc1102 282.1 3.05 33% 3.06 3.07 d*-N282   na1 YES mmc0022 umc1266 -17- d*-N282 -27- bnlg197
  P d*-N282 3L   mmc0022 285 3.05 30% 3.06 3.07          
  P d*-N282 3L   umc1266 358.3 3.06 17% 3.06 3.07          
  P d*-N282 3L   bnlg197 439.4 3.06 27% 3.06 3.07          
  P d*-N282 3L   bnlg1754 635 3.09 27% 3.06 3.07          
  S v*-N1886 3L   umc1102 282.1 3.05 29% 3.06   v*-N1886   v33,et1 YES umc1102 umc1266 -7- (v*-N1886 -0- bnlg197) -29- bnlg1496
  S v*-N1886 3L   umc1266 358.3 3.06 7% 3.06            
  S v*-N1886 3L   bnlg197 439.4 3.06 0% 3.06            
  S v*-N1886 3L   bnlg1496 638.3 3.08 29% 3.06            
329C   w*-022-15 3-   umc1102 282.10 3.05 36% 3.06 3.07 w*-022-15   y10,wlu1 YES umc1102 umc1027 umc1266 -17- (w*-022-15 -0- bnlg197)
329C   w*-022-15 3-   umc1027 348.30 3.06 17% 3.06 3.07          
329C   w*-022-15 3-   umc1266 358.30 3.06 17% 3.06 3.07          
329C   w*-022-15 3-   bnlg197 439.40 3.06 0% 3.06 3.07          
329C   w*-022-15 3-   bnlg197 439.40 3.06 0% 3.06 3.07          
  S wlu1-N28 3.07 3.08 umc1489 484.70 3.07 14% 3.07 3.08 wlu1 --   YES (wlu1 -14- umc1489)
  S v*-N1387B 3L?   umc1266 358.3 3.06 27% 3.07 3.08 v33 NEW et1,v*-N1886 YES umc1266 (bnlg197 -15- v33) bnlg1754 bnlg1496
  S v*-N1387B 3L?   bnlg197 439.4 3.06 15% 3.07 3.08          
  S v*-N1387B 3L?   bnlg1754 635 3.09 25% 3.07 3.08          
  S v*-N1387B 3L?   bnlg1496 638.3 3.08 25% 3.07 3.08          
  S w*-N5 3L   phi046 529.56 3.08 10% 3.08   w*-N5   w19,wlu1,y10,wl*-N1906 YES (w*-N5 -10- phi046) bnlg1182
  S w*-N5 3L   bnlg1182 577.89 3.09 24% 3.08            
  S wl*-N1906 3L?   bnlg1182 577.59 3.09 19% 3.09   wl*-N1906   w19,wlu1,y10 YES (wl*-N1906 -19- bnlg1182)
  S wl*-N1906 3L?   umc1140 526.30 3.08 12% 3.09   wl*-N1906   w19,wlu1,y10 YES umc1140 -12- wl*-N1906 -12- umc1594 umc1136
  S wl*-N1906 3L?   umc1594 685.10 3.10 12% 3.09            
  S wl*-N1906 3L?   umc1136 712.46 3.10 12% 3.09            
  S wl*-N1906 3L?   umc1102 282.10 3.05 26% 3.09   wl*-N1906   w19,wlu1,y10 YES umc1102 -26- wl*-N1906
408J   ra3 4-   bnlg1434 4.3 4.01 21% 4.01   ra3 --   YES bnlg1434 umc2279 (ra3 -4- cyp3) umc1276
408J   ra3 4-   umc2279 22.9 4.01 23% 4.01     --   YES  
408J   ra3 4-   cyp3 37.50 4.01 4% 4.01     --   YES  
408J   ra3 4-   umc1276 46.08 4.01 25% 4.01     --   YES  
  SP cb*-N719A 4S   bx1 62.00 4.01   4.01 4.02 cb*-N719A   wt2 YES Associated in bulks
  S spt*-N1269A 4S   umc1117 211.90 4.04 17% 4.03 4.05 spt2     YES (spt2 -17- umc1117)
  S spt*-N1620B 4S?   umc1117 211.90 4.04 17% 4.03 4.05 spt*-N1620B   spt2 YES (spt*-N1620B -17- umc1117)
  P Ysk1-N844 4.04   zp1 225.70 4.04   4.04   ysk1 --   YES Associated in bulks
  P smp*-N156A 4S   umc1451 272.50 4.05   4.05   na3 NEW   YES Associated in bulks
420D;U S yel*-8957 4L   umc1132 508.30 4.08 15% 4.08 4.09 l20 NEW   YES (l20 -15- umc1132)
  P D*-N2319 5.02   umc1679 48.00 5.00-5.01   5.00 5.01 d9 --   YES Associated in bulks
506L P br3 5-   umc1591 280.40 5.04   5.04   br3 --   YES Associated in bulks
516C P ms5 5.04 5.05 mmc0081 351.00 5.05 11% 5.04 5.05 ms5 --   YES (ms5 -11- mmc0081)
  K mn*-N1536 5L   umc1224 277.00 5.04   5.04 5.05 mn*-N1536   dek9,dek22,dek27,dek33,

prg1,ren1

YES Associated in bulks
  K sh*-N1328A 5L   umc1224 277.00 5.04   5.04 5.05 sh*-N1328A   sh4 YES Associated in bulks
  K sh*-N887A 5L?   umc1224 277.00 5.04   5.04 5.05 sh*-N887A   sh4 YES Associated in bulks
  S wl*-N1393B 5L?   umc1591 280.40 5.04   5.04   wl*-N1393B   v12,vp2 YES Associated in bulks
Pheno   ps*-85-3288-28 unpl   umc1155 370.90 5.05   5.04 5.05 ps*-85-3288-28   ps1 NEW Associated in bulks
Pheno   ps*-Mu85-3061-21 unpl   umc1155 370.90 5.05   5.04 5.05 ps*-Mu85-3061-21   ps1 NEW Associated in bulks
  S v*-N1835 5L?   umc1019 411.00 5.06   5.05 5.06 v*-N1835   v12,vp2 YES Associated in bulks
  P Hsf*-N1595 5.06   umc126a 411.00 5.06   5.06   hsf1 --   YES Associated in bulks
  K fl*-N1145A 5L   bnlg609 441.20 5.06   5.06   fl*-N1145A   dek9,dek26,dek27,dek33,

prg1,ren1

YES Associated in bulks
  S sct*-N206B 5L   bnlg118 549.30 5.07   5.07 5.08 wgs1 --   YES Associated in bulks
  S ys*-N755A 5.05 5.06 umc1225 578.20 5.08 12% 5.07 5.08 ys1 --   YES (ys1 -12- umc1225)
  S l12 6.01 6.02 umc1006  103.90 6.02 4% 6.01 6.02 l12 --   YES (l12 -4- umc1006)
  S wl*-N1848 6L?   bnlg1371 58.7 6.02 8% 6.01 6.02 wl*-N1848   l10,l15,w15 YES (wl*-N1848 -8- bnlg1371) bnlg1154
  S wl*-N1848 6L?   bnlg1154 225.8 6.05 31% 6.01 6.02          
  S pg*-N1885 6L   bnlg1154 225.80 6.05 9% 6.04 6.05 pg*-N1885   cps2,pg11 YES (pg*-N1885 -9- bnlg1154)
  S w*-N335 6L   bnlg1154 225.8 6.05 15% 6.05   w*-N335   w1,w14 YES bnlg1154 -15- w*-N335 -25- dupssr15
  S w*-N335 6L   dupssr15 371.4 6.06 25% 6.05            
    wl*-N217A 6L   dupssr15 371.40 6.06 3% 6.06   wl*-N217A   w1,w14 YES (wl*-N217A -3- dupssr15)
  S gs3-N268 6L   umc1248 422.37 6.07   6.07   gs3 --   YES Associated in bulks
Pheno K vp*-86-1407-15 unpl   bnlg2132 53.3 7.00   7.00 7.03 vp*-86-1407-15   vp9,vp*-8113 NEW Associated in bulks
Pheno K vp*-86-1407-15 unpl   umc1015 300 7.03   7.00 7.03 vp*-86-1407-15   vp9,vp*-8113 NEW Associated in bulks
    vp*-8113 unpl   o2 115.40 7.01   7.01 7.02 vp*-8113   vp9 NEW Associated in bulks
    vp*-8113 unpl   bnlg1247 164.40 7.02   7.01 7.02 vp*-8113   vp9 NEW Associated in bulks
    bu1 7.02   cyp6 158.00 7.02 0% 7.02   bu1 --   YES (bu1 -0- cyp6)
  S gl*-N212 7.02   umc1016 143.6 7.02 0% 7.02   gl1 --   YES (gl1 -0- umc1016) -8- umc1393 umc1015
  S gl*-N212 7.02   umc1393 212 7.02 8% 7.02            
  S gl*-N212 7.02   umc1015 253.5 7.03 3% 7.02            
716A S v*-8647 7L   umc1015 253.50 7.03   7.02 7.03 v*-8647   v5,v27 YES Associated in bulks
  S pg*-N590B 7L?   umc1138 201.30 7.02   7.02   pg*-N590B   l4,o5,sh6,vp9 YES Associated in bulks
  S spc*-N357A 7L   umc1001 368.30 7.03-7.04   7.03 7.05       YES Associated in bulks
  S spc*-N357A 7L   umc1125 482.51 7.04 21% 7.03 7.05 spc*-N357A   ij1 YES (spc*-N357A -21- umc1125)
  S wl*-N629A 7L   umc1295 454.50 7.04 4% 7.04   wl*-N629A   wlu2 YES (wl*-N629A -4- um1295)
  S nec*-N249A 8L?   umc1457 250 8.04 22% 8.03 8.05 nec*-N249A   nec1 YES (umc1457 -22- nec*-N249A -19- umc1858)
  S nec*-N249A 8L?   umc1858 275.5 8.04 19% 8.03 8.05          
  S wl*-N203A 8L   bnlg1863 226.3 8.04 25% 8.05 8.06 wlu3 --   YES bnlg1863 (wlu3 -13- bnlg1782) bnlg1823
  S wl*-N203A 8L   bnlg1782 382.13 8.05-.06 13% 8.05 8.06          
  S wl*-N203A 8L   bnlg1823 440.7 8.07 22% 8.05 8.06          
  S wl*-N1982 8L?   bnlg1782 382.13 8.05-8.06   8.05 8.06 wl*-N1982   wlu3 YES Associated in bulks
  S v21-N25 8.06 8.07 umc1268 452.00 8.07   8.06 8.07 v21 --   YES Associated in bulks
  P d*-N203D 8L?   gst1 517.40 8.08   8.08 8.09 d12 --   YES Associated in bulks
  P d*-N307C 8L?   umc1032   8.08   8.08   d*-N394   d12 YES Associated in bulks
  P baf1 9.02   bnlg1810 62.20 9.01 7% 9.00 9.01 baf1 --   YES (baf1 -7- bnlg1810)
  S v*-N829A 9S   bnlg1724 11.80 9.00   9.00 9.01 v*-N829A   yg2 YES Associated in bulks
  S w*-N1865 9S?   bnlg1724 11.80 9.00   9.00 9.01 w*-N1865   (like wd1) YES Associated in bulks
    yg2-N27 9S   umc1279 0.00 9.00   9.00   yg2 --   YES Associated in bulks
  S w*-N1854 9S   umc1040 17.7 9.01 0% 9.01   w*-N1854   (like wd1) YES (w*-N1854 -0- umc1040 bnlg2122 bnlg1810)
  S w*-N1854 9S   bnlg2122 21.3 9.01 0% 9.01            
  S w*-N1854 9S   bnlg1810 62.2 9.01 0% 9.01            
920F S w*-9000 9-   bnlg1810 62.20 9.01   9.01   w*-9000   \w11,pyd,l6,l7 YES Associated in bulks
  S w11 9.02 9.03 pep1 167.60 9.03   9.02 9.03 w11 --   YES Associated in bulks
  P Zb8-N1443 9.01 9.02 bnlg469a 187.60 9.03   9.02 9.03 zb8 --   YES Associated in bulks
    d*-N1803B     pep1 167.60 9.03   9.02 9.03 d*-N1803B   d3 HIT Associated in bulks
  S v*-N53A 9L   sbp4 251.00 9.04 21% 9.03 9.04 v*-N53A   v1,v15 YES (v*-N53A -21- sbp4)
  S yg*-N2021 9L?   umc1417 382.61 9.05   9.05 9.06 yg*-N2021   pg12 YES Associated in bulks
  P Rld*-N1990 9.08   bnlg619 501.70 9.07   9.07 9.07 rld1 --   YES Associated in bulks
  S l19-N425 10.00-10.03   umc1337 104.40 10.02   10.02 10.03 l19 --   YES Associated in bulks
  S ij*-N504A 10S   umc1336 178.70 10.03 2% 10.03 10.04 ij*-N504A   sr3 YES (ij*-N504A -2- umc1336)
  S gs*-N163B 10S?   umc1336 178.70 10.03 6% 10.03 10.04 gs*-N163B   gs4 YES (gs*-N163B -6- umc1336)
  S l*-N1878 10L?   umc1312 141.00 10.03   10.03   l*-N1878   l19 YES Associated in bulks
  S v*-N470A 10L   umc1336 178.70 10.03-.04 3% 10.04 10.05 v*-N470A   v18 YES (v*-N470A -3- umc1336 umc1506) umc1045
  S v*-N470A 10L   umc1506 287.50 10.05 3% 10.04 10.05          
  S v*-N470A 10L   umc1045 308.90 10.06 8% 10.04 10.05          
                               
  S v*-N470A 10L   bnlg2190 354.90 10.06-.07 11% 10.04 10.05          
  S v*-N470A 10L   umc1084 388.20 10.07 18% 10.04 10.05          
  S v*-N470A 10L   umc1640 422.50 10.07 3% 10.04 10.05 v*-N470A   v18,v29 YES v*-N470A -3- umc1640
  S w*-N24 10L   umc1061 323.60 10.04 33% 10.04 10.06 w*-N24   w2 YES (w*-N24 -33- umc1061)
  S wst*-N173B 10L?   umc1506 287.50 10.05 20% 10.05 10.06 wst*-N173B   sr2 YES (wst*-N173B -20- umc1506)
  S wst*-N548 10L?   umc1506 287.50 10.05 25% 10.05 10.06 wst*-N548   sr2 YES (wst*-N548 -25- umc1506)
X10G   v18 10L   bnlg2190 354.90 10.06   10.06 10.07 v18 --   YES Associated in bulks
  S l*-N59A 10.07   bnlg1360 408.60 10.07   10.07   l13 --   YES Associated in bulks
  S v29-N418 10L   bnlg1360 408.60 10.07   10.07   v29 --   YES Associated in bulks
  S Vsr1-N1446 10L   bnlg1360 408.60 10.07 19% 10.07   vsr1 --   YES (vsr1 -19- bnlg1360)
  S l*-N1908 10L   bnlg1360 408.60 10.07   10.07   l*-N1908   l13 YES Associated in bulks
  S pg*-N1224C 10L?   umc1640 422.50 10.07   10.07   pg*-N1224C   gs4 YES Associated in bulks
Pheno   vp*-85-3339-25 unpl   umc1038 464.00 10.07   10.07   vp*-85-3339-25   g1,v29 NEW Associated in bulks
Pheno   vp*-85-3339-25 unpl   umc1084 388.20 10.07   10.07   vp*-85-3339-25   vp13 NEW Associated in bulks
  S ppg*-N340B 1S               pg15     NO Location not confirmed by SSRs
  S pg*-N526C 1S               pg*-N526C     NO Location not confirmed by SSRs
  K o*-N1242A 2S?               o*-1242A     NO Location not confirmed by SSRs
  S w*-N346 2L               w*-N346     NO Location not confirmed by SSRs
  S v*-N735 5L               v*-N735     NO Location not confirmed by SSRs
613R S wh*-8889 9-               w*-8889     NO Location not confirmed by SSRs
920E S w*-8950 9-               w*-8950     NO Location not confirmed by SSRs
920L S ygzb*-5588 9L?               ygzb*-5588     NO Location not confirmed by SSRs
920M S wnl*-034-5 9.02 9.03             wnl*-034-5     NO Location not confirmed by SSRs
  S al*-N427B 7L?               al*-N427B     NO Location not confirmed by SSRs
  S l*-N137B 2S?               l*-N137B     NO Location not confirmed by SSRs
  S pg*-N1825 10L?               pg*-N1825     NO Location not confirmed by SSRs
  S pg*-N330A 1S?2L?               pg*-N330A     NO Location not confirmed by SSRs
  S pg*-N36A 3S?               pg*-N36A     NO Location not confirmed by SSRs
  S pg*-N380 1S?               pg*-N380     NO Location not confirmed by SSRs
  S pg*-N45A 1S?               pg*-N45A     NO Location not confirmed by SSRs
  K rgh*-N786 2L?1S?               rgh*-N786     NO Location not confirmed by SSRs
  S spt*-N537A 3S?               spt*-N537A     NO Location not confirmed by SSRs
  S v*-N142 3L?               v*-N142     NO Location not confirmed by SSRs
  S v*-N308 5L?               v*-N308     NO Location not confirmed by SSRs
  S w*-N115 10L?               w*-N115     NO Location not confirmed by SSRs

 
 
 


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