Università degli Studi di Milano

Vejlupkova’s Maize DNA preps works even using milled dry seeds

— Landoni, M; Cassani, E; Pilu, R

We are routinely using the Vejlupkova’s Maize DNA preps (MNL 77:24–25) to obtain template genomic DNA for PCR-based genotyping from leaves. We found that this fast method of extracting DNA works very well even when using about 10 mg of milled dry seeds. This method allowed us to genotype seeds obtained from F2 families that are not able to germinate (e.g., mutants like emb, emp, dek, vp, etc.).

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