MNL：81
Beijing，China
Institute of Genetics,
Chinese Academy of Sciences
RAPD analysis on the
variation and mutant types induced by space special environment in maize(zea
mays L.)
—Zeng, M.
In our previous papers, we
have observed and obtained some variation and mutant types of qualitative and
quantitative traits induced by space special environment. For example, kernel
types mutant, multi-ear mutant, ear length mutant, leaf-blade mutant, special
high plant and dwarf plant mutants, resistance to B. maydis and E. turcicum etc. The experimental results have been reported[Chinese Space Sci.
Technol. 2003,23(6):64~68;MNL:74,2~3;79,3]。
The paper deals
with the RAPD analysis primary results to the variation and mutant types
induced by space special environment.
The different
variations and mutants were used as experimental materials. They were:(1)Mut7
from U8112 and U8112CK; (2)Mut10 from Me141 and Me141CK, (3)Mut1 from Yi01-4
and Yi01-4CK, (4)XH3 from [(XH×TSPT-HZ4
Pop.)×Mut2]F2 Pop. Among
them Mut2 from Yi01-4, (5)Mut4 from Zi24 and Zi24 CK. Thirty-six Operon primers
were selected, among them 9 primers analysed (Figure 1, Table 1). Marker is
DL2000, 2.0Kb, 1.0Kb, 0.75Kb, 0.5Kb, 0.25Kb, 0.1Kb from above to below,
respectively.
It can be seen that
mutants (Mut7, Mut10, Mut1, Mut4)posses the different amplification products
from Operon primers with their CK comparison, respectively, the inbred line XH3
posses also the different amplificalson products. The results show clearly that
the changes of DNA Molecular have taken place. The present experiment is an
attempt in this aspect.
Zeng M., Institute
of Genetics, Academia Sinica, 3 Datun Rd. 100101 Beijing China; 64854896(fax),
64868597; Zengmengqian@yahoo.com.cn
Figure 1.
Amplification products from Operon primers, Opx1, Opx2,Opx5,Opx6,
Opw1,Opw2,Opw18,Opw19,OpY15,
respectively.
1.U8112CK,
2.Mut7, 3.Me141CK, 4.Mut10, 5.Yi01-4CK, 6.Mut1,
7.XHCK, 8.XH3,
9.Zi24CK, 10.Mut4.
|
Table1.Comparison
of the amplification products clearly amplified from Operon primers |
||||||||||
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
|
|
U8112CK |
Mut7 |
Me141CK |
Mut10 |
Yi01-4CK |
Mut1 |
XHCK |
XH3 |
Zi24CK |
Mut4 |
|
|
— |
More1.5Kb |
— |
|
— |
|
— |
More1.5Kb |
— |
|
|
OPX1 |
|
|
|
Less1.0Kb |
|
Less1.1Kb |
|
|
|
Less1.1Kb |
|
|
— |
|
— |
|
— |
|
— |
More1.5Kb |
— |
|
|
OPX2 |
|
|
|
|
|
|
|
|
|
Less1.1Kb |
|
|
— |
More0.5,1.5Kb |
— |
|
— |
|
— |
More1.0,1.5Kb |
— |
|
|
OPX5 |
|
Less1.1Kb |
|
|
|
|
|
|
|
Less1.1Kb |
|
|
— |
|
— |
|
— |
|
— |
More1.5Kb |
— |
|
|
OPX6 |
|
Less1.5Kb |
|
|
|
|
|
|
|
Less0.5,0.75,1.0Kb |
|
|
— |
|
— |
|
— |
More1.0,1.5Kb |
— |
More0.5,1.5Kb |
— |
|
|
OPW1 |
|
Less1.0Kb |
|
|
|
|
|
|
|
Less1.0,1.1,1.5Kb |
|
|
— |
More1.0,0.75Kb |
— |
More0.5Kb |
— |
More0.25Kb |
— |
|
— |
|
|
OPW2 |
|
|
|
|
|
|
|
Less0.35Kb |
|
Less1.1Kb |
|
|
— |
|
— |
More1.1Kb |
— |
|
— |
More1.0Kb |
— |
More0.25Kb |
|
OPW18 |
|
|
|
|
|
|
|
|
|
Less1.0,1.5Kb |
|
|
— |
|
— |
|
— |
|
— |
|
— |
|
|
OPW19 |
|
|
|
|
|
|
|
Less1.1Kb |
|
Less0.75Kb |
|
|
— |
|
— |
|
— |
More0.4Kb |
— |
More0.5Kb |
— |
More0.5Kb |
|
OPY15 |
|
|
|
|
|
|
|
Less1.75Kb |
|
|